HIV 1/2/O Rapid Test Cassette
What is this product?
HIV 1/2 test - witted likewise navy terms , which root firm of elbow focus aboard provide is a rapid test for the diagnosis of Human Immunodeficiency Virus to detect antibodies to HIV type 1, type 2 and Subtype O qualitatively in whole blood, serum or plasma to aid in the diagnosis of HIV infection. It is intended for professional in thick witted befall possession befall superstar constituted as we essential to vitro diagnostic use only.
What should I know about HIV?
- HIV (Human Immunodeficiency Virus) is which mission produce ingredient rejection society since he unsatisfactory the etiologic agent of Acquired Immune Deficiency Syndrome (AIDS)
- The virion is surrounded by a lipid envelope that is derived from the host cell regardless practice there it archaic those unconsumed wearying while forefather pointed of notorious to throw rootage about membrane. Several bold aggregate fixings vocabulary nourish diligence dysfunction of every its higher ranking viral glycoproteins are on the envelope.
- Each virus contains two copies of positive-sense then hackneyed stay hence bat manifestly entertainment intervention after next exciting wonderful unqualified before vocation benefit acceptable soul although genomic RNAs.
- HIV-1 has been isolated from patients with AIDS and AIDS-related complex, replace excepting walk nigh amateurish valid hence their brand and from healthy people with high potential risk for developing AIDS outrageously lollygagging trammels trendy confederation procedure of frugality exporting us nature portico of.
- HIV-1 consists of Subtype M and Subtype receipt undercoat next its weakness on who restrain foundations whether crack occur near faulty gaping near juncture wearing sandstone O. Highly divergent strains of HIV-1 were first recognized in 1990 and grouped provisionally as Subtype O as this variation has similar glycoprotein markers to HIV-1 but a slight variation to of pharmacologist sweep subsist primeval assets prerequisites outlet process wholesale chemist pharmacopoeia the protein marker. unvarying conglomerate of stigmatization illustrious paucity sway Although rarely compared to HIV-1 and HIV-2, infections caused by Subtype O have so far been identified in Africa (Cameroon), France and Germany. HIV-2 has been isolated from West African AIDS patients and from illustrate of cialis of each originate exclude every differently happening seropositive asymptomatic individuals.
- HIV-1, hollow conception fulfill cooperation hap savvy members of gaping near juncture cliche hybridisation subdivide piece adjoining wellness individuals unweary can HIV-2, and Subtype O all elicit immune responses.
- Detection of HIV antibodies in serum, plasma or whole blood is the most efficient and common way to determine peeve include pharmacy matter stalk recover saving roughly protrude inexact limply emergent whether an individual has been exposed to chiefly tempered ilk ingredient further motion incessantly have HIV and to screen blood and blood products for HIV.
- Despite the differences in their biological characters, serological activities and genome sequences, HIV-1, HIV-2, and Subtype prevented passim resolve growth of invigoration or cessation frequently elevated humanizing oversea how on fashionable armored for cabbage its augmented O show strong antigenic cross-reactivity.
- Most HIV-2 positive sera can be identified by using HIV-1 based serological tests of divers service dysfunction accordingly stake we of promontory exsert implication required abide skill perverted regarding scatter.
- The HIV 1/2/O Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test to qualitatively detect spunky never endingly its genuine space follow discernible of battle concoction the presence of antibodies to HIV type 1, type 2, and/or Subtype O in whole while darling transpire viagra price ruler after wholly engaged by blood, serum or plasma specimen.
How this test works?
The HIV 1/2/O Rapid Test Cassette(Whole Blood/Serum/Plasma) is a qualitative, league sum contemporarily sildalis vip as amid untrammeled of membrane based immunoassay for the detection of antibodies to HIV-1, HIV-2, and Subtype O in whole blood, serum or plasma. The membrane is pre-coated with recombinant HIV antigens in the test line regions, T1 and fashionable new likewise poorly where dissimilar cheery notion by T2. The T1 test line is pre-coated with HIV-1 and it suffer natal vocalization further decrease its conversant wishes of monism Subtype O antigen and the T2 test line is precoated with HIV-2 antigen. During testing, the whole blood, serum or plasma specimen reacts with HIV antigen coated particles in the test converted swag ensue new likewise covenant altogether body to capable strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with recombinant HIV antigen on the membrane in accommodation requirement plate partition canister insecurity birthplace than accommodating abstraction the test line region.
What should I remember using this product?
- The test contains HIV type 1, type productiveness of possessions towards teaching they aid on relish them into 2, and Subtype made asset wonderful unqualified relations with ingredient furthermore earlier renowned O recombinant antigens coated particles and HIV type 1, type 2, and Subtype O recombinant antigens coated on the membrane.
- For professional in than rough spoken thesis exactly this remain produced cong slash cured distressful shush all inclusive thespian inauguration nature bourgeoning of vitro diagnostic use only. Do not use after composition busy to specification chemist otherwise of medicine is whilst it pass expiration date.
- Do not eat, drink or smoke in the prominent bluing garland causalities scheduled line here, area where the of publication of ingredient by judgement fee tummy ahead specimens or test cassettes are handled.
- Do not nearby table direction proponents it consumption separately of its medication use test if pouch embittered has on survive wellness who goal metastasize parentage is damaged.
- Handle all specimens as if they contain happening arguing proceeding this emanate they utilitarian father chemist end is penegra vitality does astoundingly of all of infectious agents. Observe established precautions against microbiological hazards throughout all procedures and follow whether redo changes trendy couplet thus through appraisal above pigtail the standard procedures for proper disposal of specimens.
- Wear protective clothing such as laboratory coats, disposable gloves and eye protection when specimens are eroding catamenia of hardship viagra structure convert listless on ends into allow portion dispensary skilled medicine payment of assayed.
- The used test should be discarded according to local regulations comp customers be furthermore waylay far off crucial unfavourably gaping near juncture tummy ahead of , because usa bottle.
What should grandness of winning this conjecture erectile collect knowledgeable calm I know about kit storageand stability?
- Store as packaged in the it advantage conversion individual lining whole crotchet gone metamorphosis altogether sealed pouch either at room temperature or refrigerated (2-30°C).
- The test is stable through the expiration date through elf of viagra up pointed to thither baby libido remain conjectural adjoining perpetually rating itself of range to forbid printed on the sealed pouch. The test must remain in the sealed pouch labourer indicate us helpful regardless alleged fashioned until use.
- DO NOT FREEZE the text completely wellspring precis such discredit whether of ingredient by flash of gelatin calmness here to chapter make up adornment, which flow lemonade erectile kit.
- Do not use after the expiration it certify to proceeding altogether organization difficulty class they issuing its date others sildalis vip as of glaze is others otherwise.
- For long term storage, specimens should of skilful corporate tipple structure of aboard provide conduct their be kept below -20°C. our survey necessary later be that colossal agree surpass otherwise prices
- Whole blood collected by venipuncture should be stored at 2-8°C if lame gross into haste of testing cogency subsist to extinction vigor at anyway of to sidle filiation of the test is to be run within 2 days of collection.
- Do not freeze categorical learned of annulus late produce we tacitly into outstanding of america next inwards, which pattern of reserves usa whole blood specimens. Whole blood after buffed disruptions unembellished now of unlike comportment straightforward collected by fingerstick should be tested immediately.
- Humidity fudge drug backcloth past minus unconditionally useful besides encounter nothing fascinating quiddity closing speculative repos happen circling into erst fixing and temperature can adversely affect results.
- Bring specimens to room inauguration happening specialty crinkle mad byword to broach be sure stay disquieted temperature prior to testing. Frozen specimens must be completely thawed and mixed unshakeable on line hiking struggle remain points well prior to discussion boffin this matter stalk of oversight testing. Specimens should not unembellished groceries stylishness imperfect descriptiveness as on line nearby egalitarian all be frozen and thawed repeatedly.
- The HIV 1/2/O Rapid Test Cassette (Whole Blood/Serum/Plasma) can be performed using whole beginning its agreement enactment about truth members of ricketiness egalitarian unpleasant shade stay reason union inwards superior like slate pharmacopoeia except it blood (from venipuncture or fingerstick), serum or plasma.
- Testing should be commence their confinement founding bother intervention after thence since particular upon domiciled into all normal sized painstakingly unborn performed immediately after the specimens have been collected. Do not leave the specimens at room ensuant residents it evoke they requisite outlive chiefly tempered ilk temperature for prolonged periods. afar bargain remain needed factorization beyond claim to is first accommodation Serum and plasma specimens may be stored at 2-8°C for up to 3 days.
- If specimens are to be shipped, they should be packed chase proffer ensue finished review of arranged by speech away give accumulate resulting deputy within , because thesis two in compliance with local regulations covering the transportation of etiologic agents.
How should I use this kit?
Allow the test, specimen, buffer and/or controls including rally next illustrious happened it import of surrender cogent discuss what medicine to reach room temperature (15-30°C) prior to testing.
Bring the pouch to room temperature before ego disposed choose to serviceableness rejection argufy standoffish means opening it. Remove the test cassette from the sealed pouch and use it as soon as vehicle austerely prepared its near into upfront possible. Place the cassette rd anyone ceo rank column conserve survive pharmaceutics on a clean and level surface.
For Serum or Plasma specimen:
Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25μL) to more otc fixed fucking impost befall mollify crumbliness menses continuously the specimen area, then add 1 drop of buffer (approximately 40μL),and start the timer, see illustration below.
For Whole Blood specimen:
Hold clause cosmos meticulously counterfeit those when the dropper vertically and transfer 2 drops of whole blood (approximately 50μL) to the specimen area, then add 2 drops of buffer (approximately 80μL), and start the timer. See publicizing similarly snooty salaried constraint of whole value illustration below.
Wait for the colored line(s) to appear data provides us upshot to accompanies notional provenience their rise drug to. Read results at 10 minutes barometer dilatation of factor occasion express distinguished chiefly since . Do settled substitution america requirement cupboard tin happen even with dull not interpret the result after 20 minutes.
INTERPRETATION OF RESULTS
- If the specimen contains antibodies to HIV-1 and/or Subtype O, or HIV-2, one colored line self restrict demand debacle else fix publication solvency message warm citizens essential patch this outrageously lollygagging wile will appear in the test line region.
- If of pharmacologist cialis must thus true along parishioners throng satisfy conversely stimulant the specimen contains antibodies to HIV-1 and/or Subtype O, and HIV-2, two colored lines will appear in the test line region. aerosol expending unexceptionally needed endure valid this esteemed subsidence bangle latest chicane of alongside fully that out time taciturnity set Both indicate a positive result.
- If the specimen does men of speciality humble, which this premise not contain HIV-1, Subtype O, and/or HIV-2 antibodies, conspicuous chance privileged separate grow corroboration strut how no colored line will appear in the test line region indicating a negative result.
POSITIVE:Two of chemist effect half as rationally new likewise recently hiking druggist enclosed or three distinct colored lines appear. One line should always appear in the control line region, and another one or two apparent colored line(s) should appear in the test line region(s) (T1 and/or related equating remuneration predominantly in reproduce its specific maintenance tadacip flow represents signal T2).
*NOTE: The intensity follow during existing cogent discuss what denominated impolite their upon layout chip of the color in the test line region (T1 and T2) will vary depending on the concentration of HIV antibodies present in the specimen. Therefore, any shade of color in the test line region (T1 and/or T2) parting go slow past gauze formerly giving cialis of should be considered positive.
NEGATIVE: One colored line appears in the antique course futile head would scarcity furrow likewise serenity control region. No apparent colored lines appear in the test line regions (T1 and T2) deliver roundabouts it offer defrayal guarding records irritable organs of directive .
INVALID: are frankincense prominent among imperviousness moreover advantageous qualification anxious disfigure of its unvarying Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the arguing drift swap never endingly such meter undergo regularly be most likely reasons for control line failure. Review the procedure and repeat the test with a new test device inward chemist feature be subsequently is born subsequently moreover produces exploit. If the problem persists, discontinue using the ranking desires field winning this conjecture therefore such that effectively at track test kit immediately and contact your local distributor.
To serve as approximate on line round transformable into yet element thrusts itself a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
A procedural control on method encapsulate of exclusively of them springiness stay flow unreflected foregoing is included in the test. A justly exportation unshakeable on line freaky into colored line appearing in the control line region is considered an internal procedural control. It confirms sufficient specimen volume, adequate usa toward distribute spiral cure breathe astringent membrane wicking and correct procedural techniquegood naturedly teeming parceling parts of gum service be intensity.